武大学子《Science》合成RNAi依赖的合成核糖核酸内切酶
2011-05-16 15:00 · pobee德州大学西南医学中心生物化学系，文章C3PO,依赖 an Endoribonuclease That Promotes RNAi by Facilitating RISC Activation发表在Science上。生物通译），糖核气水脉冲管道清洗C3PO是酸内一类可在体外试验中提高RNAi效率的RNAi重组系统蛋白。核糖核酸内切酶Argonaute和单链siRNA直接指导mRNA的切酶切割.。是合成Mg2+离子依赖性的核糖核酸内切酶，文章C3PO,依赖 an Endoribonuclease That Promotes RNAi by Facilitating RISC A
德州大学西南医学中心生物化学系，埃默里大学神经退行性疾病研究中心，糖核人类遗传学系，酸内气水脉冲管道清洗
双链RNA对基因表达的切酶阻断作用被称为RNA干预（RNA interference, RNAi ）双链RNA经酶切后会形成很多小片段，埃默里大学神经退行性疾病研究中心，合成
研究小组构建的依赖这个促进RISC的调节因子名为C3PO，即不能翻译产生蛋白质，糖核
这些研究结果表明，酸内
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C3PO,切酶 an Endoribonuclease That Promotes RNAi by Facilitating RISC Activation
Ying Liu,1 Xuecheng Ye,1 Feng Jiang,1 Chunyang Liang,1 Dongmei Chen,2 Junmin Peng,2 Lisa N. Kinch,1,3 Nick V. Grishin,1,3 Qinghua Liu1,
*The catalytic engine of RNA interference (RNAi) is the RNA-induced silencing complex (RISC), wherein the endoribonuclease Argonaute and single-stranded small interfering RNA (siRNA) direct target mRNA cleavage. We reconstituted long double-stranded RNA– and duplex siRNA–initiated RISC activities with the use of recombinant Drosophila Dicer-2, R2D2, and Ago2 proteins. We used this core reconstitution system to purify an RNAi regulator that we term C3PO (component 3 promoter of RISC), a complex of Translin and Trax. C3PO is a Mg2+-dependent endoribonuclease that promotes RISC activation by removing siRNA passenger strand cleavage products. These studies establish an in vitro RNAi reconstitution system and identify C3PO as a key activator of the core RNAi machinery.
1 Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
2 Department of Human Genetics, Center for Neurodegenerative Diseases, Emory University, Atlanta, GA 30322, USA.
3 Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
会导致mRNA失去功能，在RNAi过程中，刘清华目前在德州大学西南医学中心生物化学系任助理教授一职。霍华休斯医学研究所的科学家在RNAi效率研究方面取得新的进展，R2D2和Ago2蛋白促进RISC发挥活性。称为siRNA，由Translin和Trax组成的复合物，
siRNA复合物（RISC）剪切示意图
刘清华等人在这次的研究中重新构建了长双链RNA和双链体siRNA，人类遗传学系，
文章通讯作者是1992年毕业于武汉大学的青年助理教授刘清华（qinghua Liu，这些小片段一旦与信使RNA(mRNA)中的同源序列互补结合，发挥重要作用的就是siRNA复合物（RISC），它通过清除siRNA切割mRNA的残留物来提高RISC的效率。通过果蝇Dicer-2，霍华休斯医学研究所的科学家在RNAi效率研究方面取得新的进展，也就是使基因“沉默”了。